Fig. 2

PCR settings and number of Hexapoda-positive samples. a Consistency/inconsistency in the detection of Hexapoda reads among the four PCR settings. The 58 samples from which Hexapoda reads were obtained in any of the four settings are shown. b Comparison of the number of Hexapoda-positive samples. In addition to the results from each single PCR condition, those based on the combination of two PCR settings are shown