Fig. 3

Propidium iodide (PI) staining of a cleared A. vulgare male. a Top view of the stained specimen. Dotted lines indicate the contour of male reproductive organs. Legs are removed. Scale bar = 2 mm. b Side view of the middle body part. Scale bar = 1 mm. Testis follicles (Tf), sperm vesicle (Sv), vas deferens (Vd). c Close-up image of the dorsal anterior hindgut. Large cell nuclei aligned in ordered rows. Scale bar = 500 μm