Fig. 1

Comparison of TR33723, TR37685, and TR38642 mRNA levels in asexual worms, sexual worms, and cocoons. qRT-PCR data are shown relative to the expression level (normalized to the Dr-ef1a mRNA level) in the asexual worm; log2 (relative expression) on the vertical axis indicates −∆∆Ct. Each circle indicates an individual asexual worm, sexual worm, or cocoon sample. The bars in the plots indicate the average − ∆∆Ct values. Asterisks indicate significant differences compared with asexual worms (Tukey’s HSD test: **P < 0.01; ***P < 0.001)