Fig. 7

Distribution of VWYa-expressing neurons in subepithelial layers of V. multiformis. a–e Subepithelial neural networks on the aboral (a, c) and oral surfaces (b, d, e) are detected by immunofluorescent staining with anti-VWYa antibody. f-f” VWYa-positive neurons (green) develop polygonal neural networks in the aboral subepithelial layer that were partially overlapped by α-tubulin staining (magenta) in a reticular pattern. g–g” VWYa-positive neural net-like structures (green) and phalloidin-positive processes (magenta) on the aboral surface. Some of these processes (magenta) extend from VWYa-positive cell bodies (green). Scale bar, 50 μm (a, b, d and f) or 10 μm (c, e, and g)