Fig. 2

Phylogenetic placement of gut symbiotic bacteria from field-collected M. japonensis adults based on 16S rRNA gene sequences. A maximum likelihood tree inferred from 1,413 aligned nucleotide sites is shown with bootstrap values of no less than 70%. The gut symbiotic bacteria of M. japonensis are colored, and the sample IDs are listed in Table S1. Asterisks denote gut symbiotic bacteria uncultivable on LB agar plates. The arrow indicates the isolated bacterial strain used in the rearing experiment. The gut symbiotic bacteria of other stinkbug species are highlighted in boldface. Brackets contain sequence accession numbers. Photos show the colony forms of gut symbiotic bacteria from each insect sample on LB agar plates