Colony start | Growth | Colony end | Observations | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Experiment 2 (27.02—22.03, 2023) | Ancestrula | Feeding ancestrula | Number of cya (s)/growing edges | No. of auto-zooids | No. of buds | Total cya growth (µm) | Days observed | Growth rate day−1 (µm) | New cya (s) added | New zooids added | No. of buds into zooids | Advanced buds | New buds | Notes on new buds and zooids | Comments on substrate | |
S2-F | ✓ | 4 | 1 | - | 452,5 | 20 | 8,6 | 1 | - | - | - | - | Secondary cystid appendage developed from ancestrula when growth halted at one end of the primary cystid appendage | Additional nutrition. Ancestrula fed throughout experiment. Growth on Ensis sp. shell | ||
S3-F | ✓ | 1 | - | - | 38,0 | 14 | 2,7 | - | - | - | - | - | Not obvious if second cystid appendage is present | Additional nutrition. Probing of tentacles observed. Growth on Ensis sp. shell | ||
S4-F | ✓ | 2 | - | 1 | 593,8 | 16 | 16,5 | 1 | - | 1 | - | - | Established bud to autozooid = 7 days | Additional nutrition. Ancestrula fed throughout experiment. Growth on Ensis sp. shell | ||
Mean = 361,4 ± SD 296 | Mean = 9,3 ± SD 7 |